rabbit anti ctip polyclonal antibody  (Bethyl)

Journal: iScience

Article Title: A bacterial genotoxin reveals a p53-proteasome-LC3 regulatory axis that drives the suppression of autophagy in cells experiencing sublethal DNA damage

doi: 10.1016/j.isci.2025.112118

Figure Lengend Snippet:

Article Snippet: Cellular lysates were fractionated by SDS-PAGE (12% gels), transferred to polyvinylidene difluoride (PVDF) membranes, and membranes were blocked with 5% non-fat milk in TBS-T. Membranes were then incubated with rabbit monoclonal anti-CtIP antibodies (1:3000 dilution in TBS-T; #9201, Cell Signaling Technology, USA), rabbit monoclonal anti-cleaved caspase-3 (1:3000 dilution in TBS-T; #9661, Cell Signaling Technology, USA), or rabbit monoclonal anti-GAPDH antibodies (1:20,000 dilution in TBS-T; #5174, Cell Signaling Technology, USA), followed by anti-rabbit IgG, HRP-linked secondary antibodies (1:10,000 dilution in TBS-T; #7074, Cell Signaling Technology, USA).

Techniques: Virus, Recombinant, Cell Recovery, Modification, Gentle, Flow Cytometry, Reverse Transcription, Bicinchoninic Acid Protein Assay, RNA Extraction, Western Blot, Cloning, Mutagenesis, Plasmid Preparation, Software, Cell Culture, Microscopy, Nucleic Acid Electrophoresis